Header Logo

Search Result Details

This page shows the details of why an item matched the keywords from your search.
One or more keywords matched the following properties of DeCoursey, Thomas
PropertyValue
overview My ORCID is 0000-0002-4263-180X. My Scopus ID is 7005381745. My NIH COMMONS name is TDECOURSEY. Research Areas: The properties and biological functions of ion channels are long-term interests of Tom DeCoursey’s laboratory. A major focus in recent years is the voltage-gated proton channel (Hv1). Modulation of the voltage-dependence of this channel by pHo and pHi ensures that it opens only when the electrochemical gradient for H+ is outward (in most species). In other words, when the proton channel opens, it extrudes acid from cells. In a long collaboration with Dr. Vladimir V. Cherny and others, the behavior of proton channels has been explored in alveolar epithelial cells and in white blood cells (human neutrophils and eosinophils). Immune cells engulf (phagocytose) bacteria and kill parasites by secreting reactive oxygen species (e.g., Chlorox). The enzyme responsible for these heroic actions is NADPH oxidase. This enzyme moves electrons across the cell membrane to form superoxide anion near the invading critters. We measure the electron movement directly as an electrical current. For each electron that leaves, one proton stays in the cell. To prevent massive depolarization as well as acidification, protons exit the cell through proton channels. Without H+ efflux, the killing process would be interrupted prematurely. Fortunately, proton channels are activated, relieving the cell of excess acid, and preventing depolarization. The discovery of proton channels has been a great boon to cells, who until this time had to use other, less efficient means of extruding acid. Identification of proton channel genes in 2006 has transformed the field. More functions are described each year, and structure-function studies are appearing. The channel was shown to be a dimer, with conduction pathway in each protomer. The dimer gates cooperatively - both protomers need to move before either conducts. New genes (over a dozen at last count) are appearing at a high rate. The proton channel resists efforts to crystallize it. The first crystal structure was reported in 2014 by Takeshita et al, is likely closed, and is of a chimera with a Voltage-Sensing Phosphatase. Hv1 triggers the flash in bioluminescent dinoflagellates (Smith et al, 2011), which were recently active in Tasmania! My Faculty Profile at Rush University Medical Center: https://www.rushu.rush.edu/faculty/thomas-decoursey-phd My Laboratory: https://www.rushu.rush.edu/research/departmental-research/physiology-and-biophysics-research/laboratory-tom-decoursey-phd http://www.phys.rush.edu/TomD/physiotd.html My NCBI Bibliography: https://www.ncbi.nlm.nih.gov/pubmed/?term=DeCoursey+TE My Scopus: https://www.scopus.com/authid/detail.uri?authorId=7005381745 My ORCID: http://orcid.org/0000-0002-4263-180X Education: PhD, University of Cincinnati College of Medicine BA, McPherson College, Kansas
One or more keywords matched the following items that are connected to DeCoursey, Thomas
Item TypeName
Concept Lymphocytes
Concept Epithelial Cells
Concept COS Cells
Concept HL-60 Cells
Concept CHO Cells
Concept B-Lymphocytes
Concept Tumor Cells, Cultured
Concept HEK293 Cells
Concept Receptors, Antigen, B-Cell
Concept Leukemia, Lymphocytic, Chronic, B-Cell
Concept Killer Cells, Natural
Concept Cells, Cultured
Concept T-Lymphocytes
Academic Article Mechanisms of potassium channel block in rat alveolar epithelial cells.
Academic Article Hydrogen ion currents in rat alveolar epithelial cells.
Academic Article Selectivity and gating of the type L potassium channel in mouse lymphocytes.
Academic Article Permeant ion effects on the gating kinetics of the type L potassium channel in mouse lymphocytes.
Academic Article Intrinsic gating of inward rectifier in bovine pulmonary artery endothelial cells in the presence or absence of internal Mg2+.
Academic Article State-dependent inactivation of K+ currents in rat type II alveolar epithelial cells.
Academic Article Electroimmunology: the physiologic role of ion channels in the immune system.
Academic Article Voltage-dependent ion channels in T-lymphocytes.
Academic Article Two types of potassium channels in murine T lymphocytes.
Academic Article Mitogen induction of ion channels in murine T lymphocytes.
Academic Article Potassium currents in rat type II alveolar epithelial cells.
Academic Article Ion channels in the metabolic regulation of respiratory cells.
Academic Article A voltage-gated potassium channel in human T lymphocytes.
Academic Article Voltage-gated potassium channels are required for human T lymphocyte activation.
Academic Article Voltage-gated K+ channels in human T lymphocytes: a role in mitogenesis?
Academic Article The voltage-activated hydrogen ion conductance in rat alveolar epithelial cells is determined by the pH gradient.
Academic Article Na(+)-H+ antiport detected through hydrogen ion currents in rat alveolar epithelial cells and human neutrophils.
Academic Article Effects of external Rb+ on inward rectifier K+ channels of bovine pulmonary artery endothelial cells.
Academic Article A scheme to account for the effects of Rb+ and K+ on inward rectifier K channels of bovine artery endothelial cells.
Academic Article Mechanism of K+ channel block by verapamil and related compounds in rat alveolar epithelial cells.
Academic Article Voltage-activated proton currents in membrane patches of rat alveolar epithelial cells.
Academic Article Ion channels in human THP-1 monocytes.
Academic Article Voltage-activated proton currents in human THP-1 monocytes.
Academic Article Proton and chloride currents in Chinese hamster ovary cells.
Academic Article HERG-like K+ channels in microglia.
Academic Article Idiosyncratic gating of HERG-like K+ channels in microglia.
Academic Article Temperature dependence of voltage-gated H+ currents in human neutrophils, rat alveolar epithelial cells, and mammalian phagocytes.
Academic Article pH-dependent inhibition of voltage-gated H(+) currents in rat alveolar epithelial cells by Zn(2+) and other divalent cations.
Academic Article Hypothesis: do voltage-gated H(+) channels in alveolar epithelial cells contribute to CO(2) elimination by the lung?
Academic Article Upregulation of Kv1.3 K(+) channels in microglia deactivated by TGF-beta.
Academic Article Absence of proton channels in COS-7 cells expressing functional NADPH oxidase components.
Academic Article The voltage dependence of NADPH oxidase reveals why phagocytes need proton channels.
Academic Article Voltage-gated proton channels help regulate pHi in rat alveolar epithelium.
Academic Article The antibacterial activity of human neutrophils and eosinophils requires proton channels but not BK channels.
Academic Article HVCN1 modulates BCR signal strength via regulation of BCR-dependent generation of reactive oxygen species.
Academic Article pH regulation and beyond: unanticipated functions for the voltage-gated proton channel, HVCN1.
Academic Article Strong glucose dependence of electron current in human monocytes.
Academic Article Construction and validation of a homology model of the human voltage-gated proton channel hHV1.
Academic Article Voltage-gated proton channels.
Academic Article Peregrination of the selectivity filter delineates the pore of the human voltage-gated proton channel hHV1.
Academic Article Enhanced activation of an amino-terminally truncated isoform of the voltage-gated proton channel HVCN1 enriched in malignant B cells.
Academic Article Tryptophan 207 is crucial to the unique properties of the human voltage-gated proton channel, hHV1.
Academic Article Altered K+ channel expression in abnormal T lymphocytes from mice with the lpr gene mutation.
Academic Article Ion channels in T lymphocytes.
Academic Article Ion channels in lymphocytes.
Academic Article Histidine168 is crucial for ?pH-dependent gating of the human voltage-gated proton channel, hHV1.
Academic Article Exotic properties of a voltage-gated proton channel from the snail Helisoma trivolvis.
Academic Article Hydrophobic gasket mutation produces gating pore currents in closed human voltage-gated proton channels.
Academic Article Expression and function of voltage gated proton channels (Hv1) in MDA-MB-231 cells.
Search Criteria
  • B Lymphocytes